In Vitro Models

Biomodels offers a full-service in vitro facility equipped with cell culture, microscopy (including immunofluorescence capabilities), cell separation system, FACS analysis and a wide variety of assays to assess drug response. Our services include, primary cell culture, cell purification and enrichment for both adoptive transfer and cancer stem cell analysis, cell based assays including proliferation and migration assays, determination of cellular fibrosis via hydroxyproline assays, quantitation of cytokine levels and other secreted protein via ELISA, western blot, quantitation of alterations in gene expression via RT-PCR and cell transfection and infection for production of viruses to generate stable cell lines.
 
 

*Representative examples of data from each assay shown below.
 
 

Cell Based Assays

Cell Proliferation and Survival


(A) MTT- proliferation/viability assays comparing breast cancer cells enriched for cancer stem cells to the parent (non-enriched) cell line. (B) Proliferation/viability of enriched breast cancer stem cells treated with a variety of experimental compounds.

Relevant Models: Oncology

 
 

Cell Migration and Invasion


(A) Invasive cell migration assays comparing breast cancer cells enriched for cancer stem cells to the parent (non-enriched) cell line. (B) Invasive migration of enriched breast cancer stem cells treated with a variety of experimental compounds.

Relevant Models: Oncology

 
 

Cell Transfection/Infection


Example of 293T cells transfected with lentiviral vectors with a GFP reporter for production of virus for subsequent infection.

 
 

Primary Cell Line Generation from Mouse Tissues


B-cell expansion of primary cells isolated from mouse bone marrow.

 
 

Molecular Biology

 

ELISA


Human normal lung fibroblasts were treated as indicated above. The results above are from an ELISA assay detecting the amount of pro collagen secreted by the cells resulting from treatment as an indicator of the development of fibrosis.

 
 

Hydroxyproline Assay

Human normal lung fibroblasts were treated as indicated above for 48 hours. Data represent the amount of hydroxyproline generated by the cells resulting from treatment as an indicator of the development of fibrosis.

Relevant Models: Fibrosis and Pulmonary

 
 

RT-PCR


 

Representative qPCR data evaluating inflammatory marker expression in diseased and naïve animals of a DSS-induced colitis model.

Relevant Model: Colitis/IBD

 
 

Cell Purification and Analysis

 

Cell Separation/Purification (Cancer Stem Cells) and FACS Analysis


 

FACS analysis of MDA-MB-231 breast cancer cells enriched for cancer stem cells using cell separation methods.

Relevant Model: Oncology

 
 

Cell Separation Purification (Adoptive Transfer Colitis Models) and FACS Analysis


Representative FACS data pre and post sorting to isolate naïve T cells for adoptive transfer in a mouse model of IBD.

Relevant Model: Colitis/IBD