In Vitro Models

In Vitro Models

Biomodels offers a full-service in vitro facility equipped with cell culture, microscopy (including immunofluorescence capabilities), cell separation system, FACS analysis and a wide variety of assays to assess drug response.  Our services include, primary cell culture, cell purification and enrichment for both adoptive transfer and cancer stem cell analysis, cell based assays including proliferation and migration assays, determination of cellular fibrosis via hydroxyproline assays, quantitation of cytokine levels and other secreted protein via ELISA, western blot, analysis of ex vivo cell populations by FACS, quantitation of alterations in gene expression via RT-PCR and cell transfection and infection for production of viruses to generate stable cell lines. Additionally, Biomodels’ microbiology facility (including anaerobic chamber), allows for the culture and dosing of aerobic and strict anaerobic bacteria. 

 

Cell Based Assays Biochemical Assays
Cell Purification & Analysis
Molecular Biology Microbiology
  • DNA isolation
  • Reverse Transcription PCR
  • qPCR
  • Sequencing
  • Transfection/ Transduction
  • Aerobic Bacterial Culture
  • Anaerobic Bacterial Culture
  • CFU determination (experimental test-article bacteria & feces): aerobic & anaerobic

 

Representative data below, click images to enlarge.

Cell Proliferation and Survival: (Left) MTT- proliferation/viability assays comparing breast cancer cells enriched for cancer stem cells to the parent (non-enriched) cell line. (Right) Proliferation/viability of enriched breast cancer stem cells treated with a variety of experimental compounds
Cell Migration and Invasion:  (Left) Invasive cell migration assays comparing breast cancer cells enriched for cancer stem cells to the parent (non-enriched) cell line. (Right) Invasive migration of enriched breast cancer stem cells treated with a variety of experimental compounds
Primary Cell Line Generation from Tissues: B-cell expansion of primary cells isolated from mouse bone marrow
ELISA:  Human normal lung fibroblasts were treated as indicated above.  The results above are from an ELISA assay detecting the amount of pro collagen secreted by the cells resulting from treatment as an indicator of the development of fibrosis.
Hydroxyproline Assay:  Human normal lung fibroblasts were treated as indicated above for 48 hours. Data represent the amount of hydroxyproline generated by the cells resulting from treatment as an indicator of the development of fibrosis.
qPCR:  Representative qPCR data evaluating inflammatory marker expression in diseased and naïve animals of a DSS-induced colitis model
Cell Purification: FACS analysis of MDA-MB-231 breast cancer cells enriched for cancer stem cells using cell separation methods
Cell Separation Purification: Representative FACS data pre and post sorting to isolate naïve T cells for adoptive transfer in a mouse model of IBD.
Representative FACS data for purification of Lamina Propria Treg Lymphocytes
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