Cell and Molecular Biology

Cancer Cell Line Inventory

Murine/Rat Cancer Cell Lines Validated In Vivo

Indication Cell Lines Readouts
Blood Cancers A20, EL4, L1210
  • Survival
  • Tumor growth kinetics (caliper/IVIS)
  • Progression/metastasis kinetics
  • Weight change
  • Immunohistochemistry
  • Immunophenotypic analysis (flow)
  • Gene expression (WB, qPCR)
  • Cytokine analysis (ELISA, qPCR etc.)
  • Mixed Lymphocyte reactions
  • Adoptive transfer
  • In vitro assays
Breast Cancer

4T1, Walker 256 (rat), *4T1-Luc

Colorectal Cancer CT-26
Brain Cancer

GL261, F98 (rat), *GL261-Luc

Lung Cancer LLC-1
Melanoma B16.F0, B16.F10, B16.F10.SIY
Sarcoma Yoshida
Treatments: Chemotherapies, Radiotherapies, Immunotherapies

*Indicates Bioluminescent Cell Lines

 

 

Human Cancer Cell Lines Validated In Vivo

Indication Cell Lines Readouts
Blood Cancers

CCRF-CEM, CEM, Daudi, Jeko-1, Jurkat, Kasumi-1, KG-1, Molt-4, Raji, Ramos, Reh, RPMI8226, THP-1, Z-138

  • Survival
  • Tumor growth kinetics (caliper/IVIS)
  • Progression/metastasis kinetics
  • Weight change
  • Immunohistochemistry
  • Immunophenotypic analysis (flow)
  • Gene expression (WB, qPCR)
  • Cytokine analysis (ELISA, qPCR etc.)
  • Mixed Lymphocyte reactions
  • Adoptive transfer
  • In vitro assays
Breast Cancer

MDA-MB-231, MDA-MB-468, *HBCx-14-Luc1, *MDA-MB-231-Luc

Colorectal Cancer

HCT-15, HCT-116, HT-29, *HCT-116-Luc, *HT-29-Luc

Brain Cancer IMR-32, U87-MG, U118-MG

Head and Neck Cancer

Cal-27, FaDu, SCC-25, SCC-154, *FaDu-Luc, *SCC-25-Luc
Liver Cancer HepG2, Hep3B
Lung Cancer

A549, H69, H82, H460, H520, H1299, H146, *H460-Luc

Melanoma

SK-MEL-28, *SK-MEL-28-Luc, *MELx-006-Luc1

Ovarian Cancer

OVCAR-3, *OVA21-DMT-Luc

Pancreatic Cancer AsPC-1, BxPC3, MIA PaCa-2, Panc-1
Prostate Cancer DU-145, PC-3
Renal Cancer 786-0, A498
Stomach Cancer N-87
Treatments: Chemotherapies, Radiotherapies, Immunotherapies

*Indicates Bioluminescent Cell Lines

MACSQuant Analyzer for FACS analysis (Miltenyi Biotec)

Ability to analyze up to seven parameters simultaneously. For use in the detailed characterization of multiple cell types, assessment of cytokine production, intra-cellular signaling, and evaluation of the purity of cell populations. Relevant to any model in which a particular cell type needs to be identified and/or characterized. For example, assessing tumor infiltrating leukocytes in oncology models, activation of lymphocytes following drug delivery, and tracking engraftment in adoptive transfer models.

MACSQuant Analyzer
Cell Separation System (Miltenyi Biotec)

Uses magnetic cell separation technology to enrich specific sub populations from a wide variety of cell types based on expression or absence of specific surface markers. For example, bone marrow preparations can be depleted of CD4+ T cells prior to adoptive transfer in GVHD models, and naïve T cells can be purified from spleen preparations for use in colitis models.

 

RT-PCR

Used commonly in molecular biology to detect RNA expression; our capabilities include PCR, RT-PCR, qPCR, and qRT-PCR.