Biomodels enters the germ-free and gnotobiotic research space!

The environmentally exposed surfaces of mammals are colonized by a diverse ecosystem of microbes. Though many of these bacteria – particularly those of the distal gut – are considered symbiotic, the microbiome has the capacity to induce both pro- and anti-inflammatory responses. Accumulating evidence suggests that a properly balanced gut microbiome is crucial for a correctly functioning immune system, and that imbalances in the microbial community of the intestine are linked to a multitude of auto-inflammatory and auto-immune disease states. When studying such disease models with a microbiome component, Germ-Free and Gnotobiotic mice provide an ideal model system.

Biomodels has 14 Germ-Free isolators (4-5 cages per isolator=20-25 mice per isolator). Isolators are maintained under strict asepsis: all items entering the isolators are sterilized by steam (autoclave) or cold (Chlorine-dioxide based) sterilization. Quality control (autoclave loads validated by spore test, three rounds of sterility conformation prior to animal arrival: isolator surface and mold trap) is built into every germ-free or Gnotobiotic study. All animal manipulation throughout the study occurs within the Germ-Free isolator, and studies are available with or without microbial monitoring (culture or 16S rRNA gene qPCR) during the in-life portion.

Our current disease areas of interest within this space are Inflammatory Bowel Disease (IBD), Graft versus Host Disease (GVHD), Oncology, Cancer Supportive Care, Gastrointestinal Acute Radiation Syndrome (GI-ARS), Experimental Autoimmune Encephalomyelitis (EAE)/Multiple Sclerosis (MS).

Should you be interested in discussing a potential project or learning about our Germ-free and Gnotobiotic capabilities please email info@biomodels.com .