Head and Neck Cancer

Head and neck cancers (of which there are many and can affect the oral cavity, the pharynx, the larynx, the paranasal sinuses and nasal cavity, and salivary glands) account for approximately three percent of all cancers in the U.S. They are twice as common among men as they are in women and it was estimated that more than 52,000 men and women would be diagnosed with head and neck cancers in 2012.

Indication Cell Line Available Models
Head and Neck Carcinoma FaDu, SCC-25 Xenograft, Orthotopic, In Vitro Assays, Cancer Stem Cells

 

Preclinical Models

Model Description Length Endpoints
Xenograft Tumor cells are implanted subcutaneously in rats or mice Variable Tumor volume, histology, cytokine induction, protein, and RNA expression.
Orthotopic Cells are implanted directly into the desired organ site in rats or mice
Cancer Stem Cells Tumor initiating cells are enriched from a heterogeneous bulk tumor cell population.  Cancer stem cells are studied in comparison to the “parent” line and can be assessed in 3-D culture in vitro or implanted into animals in vivo.  In vitro: Tumorsphere Formation, Proliferation, Survival, Migration, FACS profile.

In Vivo
: Tumor volume, metastasis, histology, cytokine induction, protein and RNA Expression
In Vitro Assays Assays for: Proliferation and survival, migration and invasion, tumorspheres/colony formation, and FACS biomarker analysis Tumor cell growth, invasiveness and survival
* Several models are compatible with IVIS imaging for in-life monitoring of disease progression.

 

Orthotopic Model of Head and Neck Cancer

cispin

 

tumorweights