Tumors that arise from astrocytes, glioblastomas are generally found in the cerebral hemispheres of the brain, but can be found anywhere in the brain or spinal cord. Highly malignant, they grow and spread aggressively, causing symptoms of headache, nausea, and drowsiness as well as cognitive impairments. They represent about 17% of all primary brain tumors.


Indication Cell Line Available Models
Glioma Carcinoma U87-MG, U118-MG, F98 Xenograft, Orthotopic, Syngeneic, In Vitro Assays, Cancer Stem Cell


Preclinical Models

Model Description Length Endpoints
Xenograft Tumor cells are implanted subcutaneously in rats or mice Variable Tumor volume, histology, cytokine induction, protein, and RNA expression.
Orthotopic Cells are implanted directly into the desired organ site in rats or mice
Syngeneic Cells are from the same species as host and are immunocompatible therefore immunocompetent animals can be used.
* Several models are compatible with IVIS imaging for in-life monitoring of disease progression.


Orthotopic/Syngeneic Model of Rat Glioblastoma


Orthotopic model of glioblastoma. Fischer rats were inoculated intracranially with 5 x 10^4 F98 cells. Animals were evaluated daily for neurological deficits. At twenty-one days, all surviving animals presented with neurological deficits (lethargy, listing, inactivity). Animals were euthanized and brains were harvested. (A) Image of rat brain containing a glioblastoma tumor (indicated by arrow). (B) Representative histological images: H & E and Luxol Fast Blue (myelin) staining.