Colorectal Cancer

Of cancers that affect both men and women, colorectal cancer is the second leading cause of cancer-related deaths in the United States and the third most common cancer in men and in women. In 2010, 131,607 people in the U.S. were diagnosed with colorectal cancer, and there were 52,045 deaths in the U.S. as a result of colorectal cancer.
 
 

 

Indication Cell Line Available Models
Colorectal Carcinoma HCT-15, HCT-116, HT-29, CT26 Xenograft, Orthotopic, In Vitro Assays, Cancer Stem Cells

 
 
 

Preclinical Models

Model Description Length Endpoints
Xenograft Tumor cells are implanted subcutaneously in rats or mice Variable Tumor volume, histology, cytokine induction, protein, and RNA expression.
Orthotopic Cells are implanted directly into the desired organ site in rats or mice
Cancer Stem Cells Tumor initiating cells are enriched from a heterogeneous bulk tumor cell population.  Cancer stem cells are studied in comparison to the “parent” line and can be assessed in 3-D culture in vitro or implanted into animals in vivo.  In vitro: Tumorsphere Formation, Proliferation, Survival, Migration, FACS profile.

In Vivo
: Tumor volume, metastasis, histology, cytokine induction, protein and RNA Expression
In Vitro Assays Assays for: Proliferation and survival, migration and invasion, tumorspheres/colony formation, and FACS biomarker analysis Tumor cell growth, invasiveness and survival
* Several models are compatible with IVIS imaging for in-life monitoring of disease progression.

 
 
 

Xenograft Model of Colorectal Cancer

 

colorectal-cancer

 
HCT-116

Above is an example of the tumor growth in a rat colorectal cancer xenograft model including standard of care. *Treated animals were dosed 3x/week (I.V.) with CPT-11 for a total of 3 weeks
 
 
 

Orthotopic Model of Colorectal Cancer

OrthotopicModelColorectalCancer

 
Colorectal tumor generated through orthotopic injection of tumor cells directly into the cecum of nude mice. Animals received either no treatment or treatment with CPT-11.
 
 
 

OrthotopicModelColorectalCancer2

 
(A) H&E staining of sections representing the cecum from untreated and (B) treated animals receiving orthotopically implanted colorectal tumor cells injected into the cecal wall. (C,D) Images and corresponding H&E staining of metastases to liver (C) and lung (D).