Additional Behavioral Tests

Biomodels offers a wide range of behavioral tests for the purposes of testing efficacy and toxicity.  Behavioral testing is useful in examining treatment for neurological disorders.  Which tests to employ are dependent on the type of disorder.  Biomodels can aid in determining which models to use and even design new models as needed.  Behavioral data is collected using automated systems and analyzed by trained scientists familiar with the models.

Behavioral tests offered:

RotaRod
Open Field
Wheel Running
 
 
 

RotaRod

Motor coordination, motor learning and physical condition are assessed by RotaRod. Animals are placed on an elevated horizontally oriented dowel. The dowel rotates at a predetermined speed, acceleration, and maximum duration or until the animal falls from the dowel. Latency to fall for each animal is measured on every trial. Performance is commonly measured over the course of multiple days with repeated trials per day.
 


Treatments that disrupt motor ability and coordination will cause impaired performance on the RotaRod. This test has been validated by examining ethanol induced ataxia. Mice were tested four trials a day over the course of two days. The graph above shows the results of the trials collapsed into two trial blocks. Mice that received ethanol fell from the RotaRod significantly faster than saline treated animals at all time points. These data demonstrate the sensitivity of the task in distinguishing motor impairments.

 
 
 

Open Field

The open field test is one of the most widely used procedures in behavioral testing. This versatile test captures measures of anxiety-like behavior and motor activity. Spontaneous activity in the open field is considered a measure of motor function. Where the animal spends time in the open field is considered a measure of anxiety. Rodents have a natural aversion to open spaces and tend to prefer the areas close to the walls of the apparatus. Anxiolytic compounds will increase time spent in the middle of the open field.
 


Treatments that stimulate or depress activity can be assessed using the open field test. This test has been validated by examining GBR-12935 induced increases in activity and diazepam induced decreases in activity. The graph above shows summed distance traveled in the open field following treatment. These data demonstrate the sensitivity of the task in distinguishing activity levels.

 
 
 

Wheel Running

Rodents show a strong circadian rhythm in voluntary wheel running behavior. Wheel running activity is monitored over several days or weeks for the purpose of assessing drug effects on exercise behavior. Wheel running measurements may be used in studies of toxicity, circadian rhythm, and voluntary exercise.
 


Plot shows mean activity in 15 minute bins over the course of 24 hours. The black bar at the top of the graph represents the dark phase of the light/dark cycle.